The two years curriculum of the MB4LS is designed to bridge the gap between data and discovery. MB4LS is designed to prepare young researchers to interact with the world’s most advanced biological and clinical datasets
Avviso di selezione n° 5/AR/2021-CIR-CNRBiOmics-IBIOM-BA per il conferimento di 1 assegno di ricerca
The aim of this project is to clarify the functional and structural relationships between respiratory complexes I and III to understand whether their supramolecular organization in supercomplexes can mitigate the effect of deleterious mutations in cytochrome b,one of the catalytic core subunits of complex III. To this purpose, biochemical approaches will be applied in human and bacterial cellular models carrying mutations in cytochrome b to analyze the structural stability and enzymatic activity of complex III in its isolated form or in assembled supercomplex I+III. The biochemical approaches will be associated with molecular dynamics simulations of supercomplex I+III by modeling wild type and mutated cytochrome b to obtain information on critical interactions between the two complexes for supercomplexes stability. From this project, we expect to obtain new knowledge on the structural and physiological relevance of supercomplexes healthy and pathological conditions.